Welcome to 13C flux world! This is the news board for 13C-MFA. We will track the recent 13C-MFA work here.

1.  Appl Environ Microbiol 2014 10.1128  The key to acetate: Metabolic fluxes of acetic acid bacteria under cocoa pulp fermentation simulating conditions. Adler et al (Wittmann group)

Authors use labeled lactate and ethanol as substrates to investigate the metabolism of acetic acid bacteria (AAB) under cocoa fermentation conditions.  They found that AAB can co-utilize lactate and ethanol simulatenously.

2. Environ Microbiol 2014 16(6), 1898-1917 Adaptation of Bacillus subtilis carbon core metabolism to simultaneous nutrient limitation and osmotic challenge: a multi-omics perspective.  Kohlstedt et al (Wittmann group)

Authors characterize the metabolism of Bacillus subtilis under nutrient limitations and osmotic stress. They found a robust carbon and energy metabolism over different tested stress conditions.

3. Microb Cell Factories 2014 13(40) Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains by Dong-Feng Liu et al (Chen Yang and Shuang-Jiang Liu group)  

Authors constructed high shikimic acid (SA) production Bacillus strains by genetic manipulation and characterize them by 13C labeling experiments. They Identify genes critical for improving SA production.

4. J Biotech 2014 (179) 42-49 13C-metabolic flux analysis in heterologous cellulase production by Bacillus subtilis genome-reduced strain by Yoshihiro Toya et al (Hiroshi Shimizu group)

Authors investigate the metabolism of cellulase production bacillus strains with reduced genome. They found PP pathway is essential for cellulase production.

5. Metab Eng 2014 (24) 173-180. Metabolic network reconstruction, growth characterization and 13C-metabolic flux analysis of the extremophile Thermus thermophilus HB8 by Aditi (Antoniewicz group)

Authors characterized a aerobic thermophilic bacteria T. thermophilus HB8. They found this bacteria had highly active glycolysis and TCA cycle fluxes and no activities of oxidative pentose phosphate pathway or Entner-Doudoroff pathway.

6.  Appl Environ Microbiol 2014 80(4):1388-1393. Deregulation of Feedback Inhibition of Phosphoenolpyruvate Carboxylase for Improved Lysine Production in Corynebacterium glutamicum by Zhen Chen et al (An-Ping Zeng group)

Authors introduced point mutations in phosphoenolpyruvatecarboxylase(PEPC) to improve lysine production in Corynebacterium glutamicum. They found mutation in PEPC effectively improve the flux of NADPH supply and thus lysine production.

7. PLOS ONE 2014 9(4) e88368. Robustness and Plasticity of Metabolic Pathway Flux among Uropathogenic Isolates of Pseudomonas aeruginosa by Antje Berger (Wittmann group)

Authors characterized a libarary of P. aeruginosa mutants. They found that all P. aeruginosa utilize Entner-Doudoroff pathway to metabolize glucose and produce high flux of NADPH to relieve oxidative stress.

8. Appl Microbiol Biotechnol 2014. Escherichia coli W shows fast, highly oxidative sucrose metabolism and low acetate formation by YalunArifin (Lars Nielsen group)

Authors characterized a sucrose utilizing E.coli strain by systems biology tools. They found a significant flux rearrangement of E. coli W strain growth in sucrose compared with growth in glucose.

9. J Biol Chem 2014 (289) 1960-1970 Non-growing Rhodopseudomonas palustris increases the hydrogen gas yield from acetate by shifting from the glyoxylate shunt to the Tricarboxylic Acid Cycle (James McKinlay group).

Authors characterize the fluxomics and transcriptiomics of Rhodopseudomonas palustris under nitrogen deprivation condition. They found non-growing cells turn to use TCA cycle to metabolize acetate and have a significant higher yield of hydrogen production (3.5 fold) compare with growing cells.

10. Metab Eng 2014 (25) 30-37. A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase. Rajesh Reddy Bommareddy et al (An-Ping Zeng group)

Authors changed the cofactor producing specificity of glyceraldehyde 3-phosphate dehydrogenase to improve lysine production in Corynebacterium glutamicum. They found the dramatic increase of lysine production has nothing with the pentose phosphate pathway.

11. Anal Chem. 2014 86(19):9423-7. Quantifying biomass composition by gas chromatography/mass spectrometry. Long and Antoniewicz

In this work, authors use 13C-biomass as internal standard to determine the biomass compositions. This work can improve flux analysis precision by quantification biomass sinks.

Updated on November, 2014